Segmentation of Axonal Fibres in Tissue Slices

This work focuses on the segmentation of axonal structures in digital images of organotypic slice co-cultures. An image processing chain is presented, which relies on anisotropic diffusion for preprocessing of the images and the intelligent scissors method for segmentation. This method requires manual user interaction to set the starting points. To overcome this drawback, the initial parameters for the intelligent scissors are automatically extracted from the images by a graph-based approach.